|
KMID : 0869020100130010035
|
|
Journal of Korean Orthopaedic Research Society
2010 Volume.13 No. 1 p.35 ~ p.42
|
|
|
Affection of Wnt/β-catenin in Titanium Particles Challenged Osteoblasts
|
|
Nam Ju-Suk
Nidi Sinha
Sharma Ashish R
Lee Jin-Koo
Kwon Sun-Chang
Chang Jun-Dong
Lee Sang-Soo
|
|
|
Abstract
|
|
|
Purpose: The intracellular mechanisms that lead to periprosthetic osteolysis including impaired bone forming activity of osteoblast remain incompletely characterized. To determine the possibility that Ti-particles play a role to regulate Wnt/β-catenin signaling pathway in impaired osteogenesis, we analyzed the stability of β-catenin and the transcriptional changes of regulators for Wnt/β-catenin signaling pathway in MC3T3-E1 osteoblast cells.
Materials and Methods: Ti-particles were prepared by sterilizing and counted on the microscopy. Transcriptional changes of OPG, RANKL, LRP5, LRP6, DKK1 and sFRP2 were determined by real-time RTPCR. Protein level of β-catenin and GSK3βwas detected using Western blotting and immunofluorescence staining.
Results: After 4 hours of treatment of Ti-particles, OPG/RANKL mRNA ratio was significantly decreased. And also, decreased protein levels of β-catenin and phospho-GSK3β were detected. Using immunofluorescence stain, it was confirmed that Ti-particles suppressed nucleus staining of β-atenin induced by Wnt3a conditioned medium. The results of real-time RT-PCR showed reduced level of LRP5 and LRP6 transcripts, and induced level of DKK1 and sFRP2 transcripts by challenging of Ti-particles
Conclusion: Our report suggests that Ti-particles may play a crucial role in the regulation of Wnt/β-catenin signaling pathway in osteoblast through the transcriptional changes of membrane receptors and extracellular inhibitors for Wnt.
|
|
|
KEYWORD
|
|
|
Wnt/ β-catenin, Osteoblast, Titanium particle
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|